EmuNations

MS-DOS Player is a MS-DOS emulator running on Windows (32/64-bit) command prompt. Its 16-bit MS-DOS compatible commands can be executed on Win32/x64 environments.

It basically inherits the environment variables from the host Windows, and a target command can access the host's file path directly. As such, you can execute 16bit MS-DOS compatible commands on your 64bit Windows in the same sence as you did on 32bit Windows, and you do not need to copy any files to/from a virtual machine (VMware, Virtual PC, XP mode, or others).

Note: This emulator DOES NOT support Win16 execution files. It aims to support character user interface utilities, for example file converters, compilers, assemblers, debuggers, and text editors. For now, doesn't support graphic/sound hardwares nor is intended to support game softwares.

VirtualBox is a powerful x86 and AMD/Intel64 virtualization solution for home and enterprise usage. It's quite feature rich, high performance yet is freely available as an Open Source Software. While it's mainly a community effort, Oracle is backing it to ensure it maintains high standards of quality.

Presently, it runs on Windows, Linux, Macintosh, and Solaris hosts and supports a large number of guest operating systems including but not limited to Windows (NT 4.0+, DOS/Windows 3.x, Linux (2.4, 2.6, 3.x and 4.x), Solaris and OpenSolaris, OS/2, and OpenBSD.

WineD3D For Windows is a DirectX 1-11 to OpenGL wrapper with almost full implementation of DirectX used in Wine. Even if Windows supports DirectX natively, using WineD3D can enhance backwards compatibility with older games, especially on Windows 8+ that don't support 16-bit screen modes. Others possible values include eliminating unsupported versions of DirectX, or porting DirectX applications to OpenGL without having to rewrite the rendering code. That said, it's far from perfect... 

Wine (Wine Is Not an Emulator) is a compatibility-layer capable of running Windows applications on several POSIX-compliant systems such as Linux, macOS & BSD.

Instead of simulating internal Windows logic like a virtual machine, Wine translates Windows API-calls into POSIX-calls on-the-fly, thus eliminating performance and memory penalties and allowing you to cleanly integrate Windows applications into your desktop.

Wine (Wine Is Not an Emulator) is a compatibility-layer capable of running Windows applications on several POSIX-compliant systems such as Linux, macOS & BSD.

Instead of simulating internal Windows logic like a virtual machine, Wine translates Windows API-calls into POSIX-calls on-the-fly, thus eliminating performance and memory penalties and allowing you to cleanly integrate Windows applications into your desktop.

DXVK provides a Vulkan-based translation layer for D3D9, D3D10, D3D11 & D3D12. It can be used on Linux with Wine to allow running 3D apps and games designed for Windows on GNU/Linux systems through Wine...

D8VK: An implementation of Direct3D 8 for DXVK. 

• Check here for me details..

Also, note Vulkan 1.3 is a firm requirement and wine 7.1 or newer.

Ludo is a minimalist frontend  launcher for most non-GL libretro cores and is written in go. It offers certain quality of life features like browsing game collections categorized by system with playlists showing in-game screenshots wrapped in a minimalist design. Also provides contextual menu which give you access to actions including quick save and quick load.

JoyToKey  enables PC game controllers to emulate the keyboard and mouse input, so that windows applications and web games can be controlled with your joysticks. Whenever buttons and sticks are pressed on the controllers, JoyToKey converts them into keyboard strokes and/or mouse movements so that the target application will work as if a real keyboard and a mouse were used.